FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16102891
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC235250.017.6298794
CCAAAGT285950.016.6104722
CGAGTAG38250.016.1162973
AAGTGCT271200.015.7333035
CAAAGTG294950.015.3900733
CCCAAAG363650.014.80229951
GCTGGGA478250.014.799349
TAGCTGG173050.014.29278857
AGTAGCT146900.014.0699485
CTACTCG39400.014.0466662
CCGAGTA46450.013.422912
CTGTCTA114500.013.206734145
GCTACTC110200.012.9747831
TACTCGG49250.012.6589543
AGTGCTG354050.012.5465726
GAGTAGC104700.012.5127334
GCTATAT58550.011.6117151
GTAGCTG188350.011.0481936
GCTACTT112750.010.5056211
GGAGGCT356900.010.5046899
Produced by FastQC (version 0.11.5)