FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15885397
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA21150.027.838521
CGTCGAG30000.020.7922212
AAAGTGC231850.017.1701224
CGAGTAG36900.016.524273
CCAAAGT282750.015.7647342
GTCGAGA40850.015.4412542
CCCAAAG356350.015.1260961
CTACTCG39550.015.062762
AAGTGCT270400.014.8261015
GCTACTC105950.014.0914561
CAAAGTG302800.014.0495433
GACTTGT136150.013.8987443
GCTGGGA468100.013.8349569
AGTAGCT148900.013.650265
CCGAGTA46450.013.4287772
TAGCTGG176450.012.6709567
ACTTGTG157750.012.3511554
TACTCGG48950.012.313323
GTGATCA157750.012.1292798
CGAGACT81150.012.0925191
Produced by FastQC (version 0.11.5)