FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16226056
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC235450.017.8555534
CTACTCG40850.017.8361782
CCAAAGT284150.015.8781062
AAGTGCT274050.015.4695775
CAAAGTG293850.015.1636583
GCTACTC106050.014.4728681
CCCAAAG356750.014.4588921
GCTGGGA483500.014.2626329
TACTCGG49050.014.1406293
CGAGTAG37550.013.993423
AGTAGCT146450.013.6366715
TAGCTGG173850.013.1005537
CTGTCTA120550.012.957688145
GAGTAGC101700.012.4703424
AGTGCTG347100.012.2949736
CCTATAT65100.011.6269011
CCGAGTA47100.011.4532922
GCTAATT115750.010.7170051
GTAGCTG190100.010.5057646
GGAGGCT365350.010.3390529
Produced by FastQC (version 0.11.5)