FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15978872
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG27250.024.1910551
ACGTCGA21650.023.3221241
CTACTCG39050.017.9558452
AAAGTGC237350.017.5474174
CCAAAGT283250.016.041022
TACTCGG47950.015.792463
AAGTGCT279300.015.2884615
GTCGAGA44100.014.9456972
CAAAGTG297500.014.9422823
GCTGGGA475750.014.8856139
CGAGTAG39200.014.8459043
CCCAAAG355000.014.61829951
GCTACTC109000.014.4760311
CGAGACT83100.014.0087871
AGTAGCT149150.013.7740545
TAGCTGG173350.013.3476487
CCGAGTA49650.012.9925822
GACTTGT141550.012.3340043
AGTGCTG348850.012.2201956
ACTTGTG161050.011.4501464
Produced by FastQC (version 0.11.5)