FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15978872
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG40450.018.7104022
AAAGTGC231950.017.3741264
CCAAAGT290400.015.419922
AAGTGCT271800.014.9067875
TACTCGG49850.014.7616413
CCCAAAG363550.014.4967161
CGAGTAG37300.014.46746353
GCTGGGA469350.014.3999849
GCTACTC109050.014.33161451
CAAAGTG296950.014.27853
TAGCTGG168250.013.7499117
AGTAGCT145100.013.4302195
GCTATAT62400.012.6914071
CCGAGTA47100.012.6466612
GCTAATT118950.012.1372061
GAGTAGC101750.012.0540554
AGTGCTG352150.011.665016
GTAGCTG181950.011.0957236
CTGTCTA117450.010.873033145
GCTACTT113900.010.3986971
Produced by FastQC (version 0.11.5)