FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19072580
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA25250.021.551011
AAAGTGC250150.016.9205514
CGTCGAG37400.015.4929812
CCAAAGT307450.015.4220032
CAAAGTG318850.014.1834453
CCCAAAG409100.014.09611
AAGTGCT307600.013.8292775
GCTGGGA566950.012.600839
GTCGAGA48200.012.4613362
GCTATAT67550.011.7173921
CTACTCG45400.011.5177762
GCTAATA60650.011.3026231
CCTAATA71250.010.9105371
TAGCTGG190050.010.8567777
AGTAGCT158750.010.7718665
AGTGCTG418900.010.6608136
TACTCGG55300.010.4779643
GCTATAC47900.010.475171
CCTATAT66850.010.2543611
GCTAATT108850.010.1282511
Produced by FastQC (version 0.11.5)