FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19337472
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC247350.016.3634154
CCAAAGT308850.014.7747682
CCCAAAG413100.013.892251
AAGTGCT301200.013.7674925
CAAAGTG321650.013.4607033
GCTGGGA565750.012.0766549
CTACTCG44950.011.9431712
GCTATAT68150.011.9262451
CGAGTAG42300.011.6882233
AGTGCTG405300.011.2948726
CCTATAT70000.011.2071891
TACTCGG55450.010.6996243
GCTAATT113900.010.6107021
GCTATTT142500.010.4650031
TAGCTGG192950.010.3619227
AGTAGCT158450.010.254335
CTATATG71950.010.0139442
GCTATAA79200.09.9945811
GCTACTC121100.09.9798431
GCTATAG68850.09.7519481
Produced by FastQC (version 0.11.5)