FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19072580
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC251750.016.4384884
CCAAAGT309800.014.6801782
AAGTGCT301750.014.068495
CAAAGTG320750.013.870633
CCCAAAG410650.013.5367041
CTACTCG47400.013.1108612
GCTGGGA558300.012.578549
GCTATAT68550.012.4677451
GCTAATA63300.012.3859481
GCTAATT108950.012.3178921
GCTACTC120550.012.1872631
AGTGCTG404350.011.1281166
CCTATAT67150.010.834311
CCTAATA68700.010.3842391
TACTCGG57250.010.3615843
AGTAGCT152900.010.30190855
TAGCTGG184900.010.1243697
CCTATTA72650.09.9168691
CGAGTAG43250.09.7968723
GCTATTA72000.09.712091
Produced by FastQC (version 0.11.5)