FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19522067
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC264650.016.5885454
CCAAAGT318150.015.77672
CAAAGTG331450.014.7170143
AAGTGCT312250.014.1731035
CCCAAAG424600.013.9231071
GCTATAT67600.012.4483031
GCTGGGA580850.012.4010929
CTACTCG42450.012.3237752
CCTATAT72900.012.1252981
AGTGCTG424600.011.2888446
CGTCGAG31900.011.0838141
GCTAATT116750.011.0236241
TAGCTGG193650.010.6227627
AGTAGCT158700.010.602135
GCTACTC122800.010.2501811
GACTTGT149050.010.2449643
GCTATTA76100.010.2215781
GATATAT92750.09.7590141
CGAGACT81750.09.5151331
CTATATA77450.09.492962
Produced by FastQC (version 0.11.5)