FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20265415
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG58100.019.2548032
CGAGTAG56000.018.4923923
AAAGTGC317100.018.4187744
CCAAAGT375950.017.5417752
AGTAGCT212950.017.5078035
TAGCTGG254050.017.014917
GCTGGGA608500.016.954839
TACTCGG68300.016.5772693
CCGAGTA66900.016.4123672
CAAAGTG389200.016.35493
AAGTGCT363650.016.2709455
CCCAAAG457700.016.1117631
GTAGCTG258150.014.9522166
GAGTAGC150350.014.9233564
GCTACTC154050.014.5807941
AGTGCTG437050.013.7926926
TTGGGAG368800.013.7613916
GCTATAT83450.012.754221
GGGAGGC563300.012.6512278
GGAGGCT469650.012.4835139
Produced by FastQC (version 0.11.5)