FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20325779
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC318600.018.826594
CGAGTAG55550.018.751253
CTACTCG57650.017.7062442
AGTAGCT213550.017.5814675
CCAAAGT383550.017.1894742
CCGAGTA62750.017.1585222
AAGTGCT356850.016.7713665
TAGCTGG249600.016.5308727
GCTGGGA633000.016.4632599
CCCAAAG470150.016.1683921
CAAAGTG396650.015.98555853
GCTACTC156450.015.1221861
GAGTAGC147750.015.0463024
TACTCGG66400.014.7396773
GTAGCTG258400.014.2078176
AGTGCTG436550.013.8721716
GCTATAT83400.013.2606391
TTGGGAG376200.012.9746946
CCCGAGT104200.012.6288431
GGAGGCT490350.012.4663279
Produced by FastQC (version 0.11.5)