FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21194784
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA24300.023.3383661
CGAGTAG60700.020.6470553
AAAGTGC340800.019.7844774
CCAAAGT414200.018.6277832
AGTAGCT232150.018.186395
GCTGGGA677900.017.6511619
AAGTGCT389950.017.5960435
CCCAAAG508050.017.3918061
TAGCTGG271700.017.1882637
CAAAGTG427250.017.0429613
CTACTCG59100.016.82252
CCGAGTA75100.016.781172
CGTCGAG34150.016.6068041
GAGTAGC164050.015.6207814
TACTCGG70150.015.3704593
GCTACTC165950.015.2307641
GTAGCTG277450.015.1665086
AGTGCTG470350.014.6030546
CCTATAT92500.013.1704091
CCCGAGT116800.013.0678881
Produced by FastQC (version 0.11.5)