FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20820138
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC332200.020.1544024
CGAGTAG58850.019.118243
CCAAAGT394200.018.6200122
CTACTCG55850.017.773482
AGTAGCT222250.017.769255
AAGTGCT383300.017.761625
GCTGGGA658950.017.2213959
CCGAGTA67600.017.1659852
CAAAGTG411650.017.1289833
CCCAAAG480900.017.1125641
TAGCTGG269000.016.6333567
GCTACTC160700.015.3716831
GAGTAGC153650.015.328564
TACTCGG70350.014.9996593
AGTGCTG465900.014.8389286
GTAGCTG275450.014.4907986
TTGGGAG383450.013.5267716
GGAGGCT487350.012.66840659
CCTATAT89500.012.6664391
GTGCTGG600850.012.5198297
Produced by FastQC (version 0.11.5)