FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20325779
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA24950.024.1138761
AAAGTGC327100.020.0619034
CGTCGAG36000.019.0441991
CGAGTAG58800.019.036693
CCAAAGT393350.018.4079152
CCGAGTA66900.017.7773192
AAGTGCT374450.017.524995
CAAAGTG401400.017.4638163
GCTGGGA647600.017.2091669
AGTAGCT225900.017.1880055
CCCAAAG484850.016.95391
CTACTCG55550.016.4979762
TAGCTGG260400.016.2810157
TACTCGG65500.014.7396293
GTAGCTG270900.014.6944416
AGTGCTG461600.014.595216
GAGTAGC152500.014.4048794
GTCGAGA50400.014.1583642
GCTACTC156550.013.9871881
CCCGAGT111900.012.5662341
Produced by FastQC (version 0.11.5)