FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19392716
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG54800.020.1406082
AAAGTGC296550.018.4871754
AGTAGCT198900.018.0570875
CGAGTAG54450.017.2991853
CCAAAGT350250.016.799672
AAGTGCT340150.016.5923025
TACTCGG66500.016.3843333
GCTGGGA605400.016.0143369
GCTACTC149900.015.9550261
CCGAGTA64000.015.7075882
TAGCTGG240000.015.69726857
CCCAAAG443500.015.6229421
CAAAGTG372150.015.3564653
GAGTAGC139700.015.0938054
GTAGCTG253400.013.86831956
AGTGCTG440500.013.1474566
TTGGGAG342350.012.6670446
GGAGGCT469400.012.5063469
CCCGAGT104100.012.027891
GCTACTT145700.011.6353031
Produced by FastQC (version 0.11.5)