FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19187472
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA25350.023.0348031
CGTCGAG34800.020.8229541
AAAGTGC293050.018.672834
CGAGTAG52400.018.6575033
AGTAGCT205250.017.4424275
CCAAAGT354150.017.4179082
CTACTCG53650.016.650132
TAGCTGG238800.016.4353947
GCTGGGA613100.016.3708279
AAGTGCT334300.016.368755
CCCAAAG434100.016.1742861
CAAAGTG370800.016.1420943
GAGTAGC139100.015.9783614
GTCGAGA47700.015.6340621
GACTTGT173950.015.3244473
CCGAGTA63800.015.1037042
GCTACTC146850.014.9473761
TACTCGG60750.014.5879513
GTAGCTG247750.014.5358716
ACTTGTG199450.013.8237014
Produced by FastQC (version 0.11.5)