FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19169987
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC291350.018.3336834
CTACTCG55300.018.3066882
CCAAAGT347050.016.8540272
GCTACTC147800.016.4636881
AGTAGCT197600.016.2545415
AAGTGCT342250.015.9559645
TACTCGG65050.015.8826683
GCTGGGA604200.015.764089
CGAGTAG54950.015.7322493
CCCAAAG434200.015.7273251
TAGCTGG234450.015.4103597
CAAAGTG371350.015.4061593
CCGAGTA63250.015.1164842
GAGTAGC139350.014.8787414
AGTGCTG421250.013.6652936
TTGGGAG341300.013.5507886
GTAGCTG249950.013.0197546
GCTACTT146500.012.8173751
GGAGGCT461400.012.446719
CCCGAGT106450.011.6937291
Produced by FastQC (version 0.11.5)