FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19400940
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG53300.019.135622
AAAGTGC299050.018.8874554
AGTAGCT200450.017.3349955
CCAAAGT360000.017.2332742
GCTGGGA601700.016.6811779
AAGTGCT344800.016.5038075
CGAGTAG53900.016.4430943
CAAAGTG376300.016.2811723
TACTCGG64900.016.1489143
CCCAAAG444050.016.0046751
GCTACTC151900.015.9352181
CGAGACT94500.015.9345321
TAGCTGG237100.015.81218057
GACTTGT163900.015.7932183
CGTCGAG29900.015.5320971
GAGTAGC136200.014.5121224
ACTTGTG192100.014.3535864
CCGAGTA64700.014.2419272
GTAGCTG248050.014.1218946
GTGATCA190900.013.7063198
Produced by FastQC (version 0.11.5)