FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19392716
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG52850.019.5587773
AAAGTGC300450.019.0043584
AGTAGCT207800.018.4426175
CTACTCG55750.018.2891062
CCGAGTA64850.017.891362
CCAAAGT360100.017.69192
GCTGGGA605650.017.2632589
TAGCTGG246050.016.9744857
AAGTGCT347850.016.7988915
CGTCGAG29500.016.688751
CCCAAAG453850.016.4108621
CAAAGTG380550.015.9281333
GACTTGT169200.015.5016463
GCTACTC148200.015.4709571
GAGTAGC146400.015.4662194
TACTCGG65900.015.0453753
GTAGCTG251900.014.9068096
ACTTGTG190200.014.788324
CGAGACT96150.014.0442871
GTGATCA193500.013.8081718
Produced by FastQC (version 0.11.5)