FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19400940
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC298300.019.00064
CTACTCG55000.018.6695562
CCAAAGT359550.017.8198012
AGTAGCT201100.017.69415
TAGCTGG236400.017.1943177
AAGTGCT340150.016.9524865
CGAGTAG53250.016.903083
GCTGGGA618150.016.5106989
CCCAAAG448150.016.4067941
CAAAGTG371900.016.3933753
TACTCGG67850.016.2714143
CCGAGTA61800.015.8186742
GCTACTC145900.015.6732441
GAGTAGC139350.015.5932654
GTAGCTG249150.014.027856
AGTGCTG426750.013.8750376
TTGGGAG353050.012.4292246
GGAGGCT468150.012.2752119
CCCGAGT107850.011.6126321
GTGCTGG565700.011.4741397
Produced by FastQC (version 0.11.5)