FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19187472
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC297250.019.6381444
CCAAAGT361050.018.6219732
AAGTGCT336000.017.4574185
CTACTCG53000.017.2505382
CAAAGTG369150.017.1655243
GCTGGGA603200.017.0823969
CCCAAAG450800.016.9947131
AGTAGCT195800.016.7748055
TAGCTGG235150.016.0317947
TACTCGG63300.014.8879963
AGTGCTG430800.014.6607126
GCTACTC146750.014.4297311
CGAGTAG53650.014.4197623
CCGAGTA57850.014.3454382
GAGTAGC138600.013.802254
GTAGCTG248600.013.7212926
TTGGGAG349000.013.3409576
GGAGGCT469300.012.5743039
GCTACTT142800.012.1685451
GTGCTGG568850.011.9190497
Produced by FastQC (version 0.11.5)