FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20613685
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG60900.020.8681683
GACTTGT175900.020.6594943
CCGAGTA69350.019.2170282
AAAGTGC366750.019.0306874
ACTTGTG205400.018.7960594
AGTAGCT238550.018.6318425
TGATCAA203650.017.9456929
CCAAAGT425150.017.7092132
CGAGACT103250.017.6313551
GCTGGGA676600.017.3415899
AAGTGCT404750.017.1591285
GTGATCA217000.017.0947538
GTCGAGA44300.017.0576341
CTACTCG58050.017.0408482
TAGCTGG279800.016.8424477
CAAAGTG443100.016.573183
CCCAAAG523500.016.3111421
GAGTAGC166950.016.2944494
GTAGCTG277350.016.1004186
GAGACTT241900.016.0167031
Produced by FastQC (version 0.11.5)