FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20524848
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG62550.022.071483
GTCGAGA45100.021.4771251
CCGAGTA70500.020.4608442
AGTAGCT233450.019.741965
GACTTGT185500.019.5502683
AAAGTGC364150.018.3146444
TGATCAA203100.017.7886859
GAGTAGC162500.017.7100664
TAGCTGG278250.017.6004357
CCAAAGT423200.017.545812
ACTTGTG210500.017.5219214
GCTGGGA697200.017.23069
GTAGCTG274850.016.868816
GTGATCA214450.016.6870548
CTACTCG57650.016.6810022
AAGTGCT408300.016.6371365
CGAGACT111950.016.4453951
GAGACTT232950.016.1308861
GCTACTC164000.015.791781
CAAAGTG450000.015.751793