FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20539500
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG38500.024.9980931
CGAGTAG60300.020.0625083
CTACTCG56900.019.5698412
GTCGAGA50450.019.0744252
AAAGTGC375100.018.4716844
AGTAGCT233550.018.453725
GACTTGT188250.018.256813
CCGAGTA69900.017.7986072
GCTGGGA687400.017.6395029
CCAAAGT428800.017.408432
AAGTGCT416950.016.8980315
TACTCGG67900.016.80463
ACTTGTG216300.016.7156124
TGATCAA209600.016.6597969
TAGCTGG274950.016.5750947
GCTACTC158850.016.4018021
CAAAGTG453400.016.3428253
GTGATCA216400.016.0409988
GAGTAGC161950.015.958794
CCCAAAG531350.015.7711221
Produced by FastQC (version 0.11.5)