FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21046817
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC338750.017.6028864
CGAGTAG60400.017.4356063
CCAAAGT399350.016.6432572
AGTAGCT223100.016.3807165
GTCGAGA51500.016.250971
CTACTCG57150.016.2305982
CCGAGTA72000.016.079582
CGTCGAG38300.016.0246731
TAGCTGG268200.015.60018357
AAGTGCT385900.015.4522785
CAAAGTG419600.015.2416123
GCTGGGA721450.015.0307979
CCCAAAG510550.014.67139151
GAGTAGC160150.014.3709034
GACTTGT179000.013.9874483
CGAGACT106150.013.929041
GTAGCTG278750.013.3357556
TGATCAA189700.013.1605359
GCTACTC157400.013.1157251
ACTTGTG207450.012.943284
Produced by FastQC (version 0.11.5)