FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20850111
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA28100.022.3311581
CGTCGAG36950.018.8695091
CGAGTAG60650.018.3933183
AAAGTGC346250.017.619324
AGTAGCT222650.016.972935
CCAAAGT406100.016.9283562
CCGAGTA70700.016.0745582
AAGTGCT399650.015.4224255
CAAAGTG425100.015.3024663
TAGCTGG264450.015.2660517
GCTGGGA725150.015.1922849
CTACTCG57100.015.1410672
CCCAAAG515150.014.9285231
TACTCGG67800.014.6025773
GAGTAGC160600.014.41330054
GTCGAGA54900.014.0969671
GACTTGT177250.014.0034373
GCTACTC154500.013.7640551
GTAGCTG275500.013.641176
TGATCAA189650.013.0150019
Produced by FastQC (version 0.11.5)