FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20850111
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC344400.019.3303344
CCAAAGT406750.017.8430962
AAGTGCT396450.017.3033335
CGAGTAG53200.017.1652223
CTACTCG58500.016.4463792
CAAAGTG422750.016.3080653
CCCAAAG516400.016.0524351
AGTAGCT213000.015.6448495
GCTGGGA716150.015.4402589
TAGCTGG264700.014.7230797
CCGAGTA64900.014.50227452
TACTCGG71100.014.3143953
AGTGCTG500200.014.1746666
GCTACTC156300.013.4821361
GCTAATT165350.013.1240221
GAGTAGC152750.012.7327464
GTAGCTG267000.012.5333046
GCTATAT69250.011.5874751
GTGCTGG654150.011.4783427
TGCTGGG746450.011.161428
Produced by FastQC (version 0.11.5)