FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16086455
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC251150.017.8803564
CGTCGAG26350.016.407611
CCAAAGT295850.016.1687362
CGAGACT83050.015.869251
CGAGTAG39200.015.65381053
AAGTGCT289500.015.4394895
TGATCAA135700.015.3590719
GACTTGT139850.015.3073263
AGTAGCT153200.015.2933985
CAAAGTG312950.014.8173373
TAGCTGG186650.014.7517217
CCCAAAG386050.014.3420491
GCTGGGA541550.014.1717139
GTGATCA157200.013.6131758
ACTTGTG162150.013.5461864
GTCGAGA39100.013.3757691
GAGTAGC109550.013.1122834
GTAGCTG202150.012.590886
TTGTGAT176150.012.5887166
CCGAGTA48950.012.535842
Produced by FastQC (version 0.11.5)