FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15847931
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG41450.017.9966953
AAAGTGC249600.017.0660974
CCAAAGT298200.015.4296982
CCGAGTA50750.015.2478322
AAGTGCT289450.015.2225235
AGTAGCT149100.015.1499245
CAAAGTG306100.014.5536193
TAGCTGG181750.014.001927
GCTGGGA536100.013.92894659
GAGTAGC106450.013.6224514
CCCAAAG392200.013.597571
CTACTCG39050.012.3182552
CCTATAT54850.012.0740911
AGTGCTG376500.011.907056
GTAGCTG192900.011.7826656
GCTACTC105100.011.2759641
GCTAATT110250.011.0653951
CCCGAGT91500.010.6663161
GCTATAG51300.010.4635931
TACTCGG50300.010.3950553
Produced by FastQC (version 0.11.5)