FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15947353
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA22500.020.7663861
CGTCGAG33150.020.4059521
CGAGTAG41800.017.3468063
AAAGTGC252700.017.1060164
CCAAAGT291900.016.313572
GTCGAGA42800.016.289912
AGTAGCT151450.015.4219045
CGAGACT85700.015.2983881
AAGTGCT286500.015.2339355
CAAAGTG307350.014.9264023
GACTTGT137450.014.8622763
CCCAAAG389650.014.3896431
TGATCAA143400.014.1491649
GCTGGGA539850.013.961749
TAGCTGG183350.013.9563587
CTACTCG37500.013.5723182
CCGAGTA53600.013.5279192
ACTTGTG160550.013.3318534
GTGATCA163950.012.5883048
GAGTAGC111400.012.5797874
Produced by FastQC (version 0.11.5)