FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15947353
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC245050.018.0613174
CCAAAGT292400.016.4930342
CTACTCG40800.016.3976972
AAGTGCT286600.015.5658315
CAAAGTG305500.014.8059153
CCCAAAG389000.014.628641
GCTGGGA534400.013.8043039
AGTAGCT142350.013.4661625
GCTAATT107200.013.0106391
TACTCGG50900.013.0079133
TAGCTGG175750.012.972247
GCTACTC111450.012.7647861
AGTGCTG381750.012.1624086
GCTATAT49650.011.5174941
CGAGTAG39800.011.3823543
GAGTAGC104450.011.0772024
GTAGCTG191300.010.7505686
CCGAGTA49750.010.2259022
GCTACTT107500.010.1848491
GCTATAA62850.09.9862091
Produced by FastQC (version 0.11.5)