FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15847931
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG40100.018.0800633
AAAGTGC247200.016.4693074
GTCGAGA39500.016.4216751
GACTTGT135150.016.0934643
CCAAAGT290700.015.6115332
AGTAGCT147400.015.3708125
CCGAGTA49700.015.28902152
TGATCAA140850.015.2448329
CGTCGAG31650.015.2057191
ACGTCGA22350.014.9794311
ACTTGTG156950.014.7464574
CAAAGTG303750.014.5277513
CGAGACT81700.014.5130421
AAGTGCT288100.014.0828545
TAGCTGG183650.013.9696987
GCTGGGA541850.013.7668169
CCCAAAG385700.013.6168591
GTGATCA161450.013.4724538
CTACTCG40900.013.4652172
GAGTAGC107050.013.1545394
Produced by FastQC (version 0.11.5)