FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16170864
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG38050.017.217342
AAAGTGC252350.016.9858594
AGTAGCT150300.016.4157735
CCAAAGT300200.015.8795532
AAGTGCT296150.014.7561435
TAGCTGG184100.014.6528217
GCTGGGA544400.014.4437259
CGAGTAG40550.014.4374563
CAAAGTG315100.014.3990843
CCCAAAG396650.014.2841971
GCTACTC111050.012.9277041
TACTCGG50200.012.7728073
GAGTAGC109050.012.65479854
CCGAGTA51400.012.47433952
GTAGCTG198100.012.1035056
AGTGCTG385650.011.9466286
GCTAATT115900.011.8455581
CCTATAT56600.011.4508911
CAAGTAG101700.09.8683333
GTGCTGG503700.09.8392437
Produced by FastQC (version 0.11.5)