FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18888448
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC313400.019.2951094
CCAAAGT361800.017.9313032
AAGTGCT361150.017.303535
CAAAGTG387300.016.3667663
CCCAAAG462950.016.0385421
GCTGGGA636750.015.7924929
AGTAGCT192000.015.5489865
CGAGTAG51000.015.280583
TAGCTGG232500.014.8460347
CTACTCG54250.014.2425482
AGTGCTG453950.014.1801156
GAGTAGC136800.013.7850924
CCGAGTA58600.013.7792272
GCTACTC140950.013.1943481
GTAGCTG240250.012.9186616
TACTCGG64600.012.6021693
GTGCTGG572500.012.1069757
GCTAATT157800.011.1674821
TGCTGGG671700.011.1270678
GCTACTT131550.011.1191141
Produced by FastQC (version 0.11.5)