FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19069420
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC314950.018.949454
CCAAAGT363750.018.0743082
CGAGTAG51850.017.9975533
AAGTGCT361700.016.4426335
CAAAGTG387250.016.2387943
CCCAAAG469550.016.1292291
AGTAGCT195800.015.5072855
CCGAGTA62500.015.3777152
GCTGGGA642500.015.30577859
CTACTCG52950.014.8629572
TAGCTGG236050.014.5453147
TACTCGG61750.014.4354533
GCTACTC144500.014.3685951
AGTGCTG448800.013.6240756
GAGTAGC142800.013.5573254
GTAGCTG243750.012.6282456
CCTATAT73600.011.92775251
GTGCTGG584250.011.634067
GGAGGCT470850.011.3006829
GCTACTT138700.011.0512681
Produced by FastQC (version 0.11.5)