FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18734600
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG50150.019.844913
AAAGTGC305700.018.7368494
CCAAAGT362750.017.635242
CCGAGTA59350.016.8894332
AAGTGCT347950.016.78225
CTACTCG51900.016.4972322
CAAAGTG376200.016.3722553
AGTAGCT189400.016.168755
CCCAAAG462150.015.6333451
GCTGGGA630600.015.5413389
TAGCTGG231200.015.2331477
GAGTAGC136150.014.4664174
TACTCGG63450.013.8204753
GTAGCTG235450.013.7755326
AGTGCTG437000.013.4584936
GCTACTC139800.012.5090021
CCCGAGT105750.012.4519551
GTGCTGG570450.011.1886767
GCTAATT160300.011.0831391
GGAGGCT465000.010.79259
Produced by FastQC (version 0.11.5)