FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19146570
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG52650.018.7789133
AAAGTGC316700.018.2923454
CTACTCG50100.017.2357522
CCAAAGT369400.017.1927052
AGTAGCT196200.017.1060645
CCGAGTA63200.016.748382
AAGTGCT364750.016.302895
GCTGGGA641700.016.266049
CAAAGTG382150.016.1246363
CCCAAAG471500.015.4419111
TAGCTGG241400.015.2879267
GAGTAGC140450.015.0711354
GCTACTC138850.014.5016821
TACTCGG61450.014.3900523
AGTGCTG449950.013.7266586
GTAGCTG246900.013.678116
GCTAATT165500.012.1665161
CCCGAGT107150.012.0294561
GTGCTGG576250.011.6486637
GGAGGCT465900.011.3289269
Produced by FastQC (version 0.11.5)