FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19567432
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC275850.018.5716114
CCAAAGT325550.017.1572132
AAGTGCT321900.015.8498915
CAAAGTG341200.015.4238333
CCCAAAG449450.014.2601481
AGTAGCT172550.013.7661465
CGAGTAG55600.013.2688723
GCTGGGA665000.013.0751749
TAGCTGG219200.012.4732177
AGTGCTG439000.012.2310856
GCTAATT126550.011.8839891
CCGAGTA66050.011.5944722
GAGTAGC131550.011.4318884
CTGTCTA135200.011.374232145
CTACTCG52700.011.0653412
CCTATAT65000.010.5955461
GCTACTC131400.010.4291721
GTAGCTG237500.010.3571586
GCTATAG59300.09.83634951
GCTATAA76500.09.7376691
Produced by FastQC (version 0.11.5)