FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19801990
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC287800.018.432624
CCAAAGT334150.017.0540622
AAGTGCT339000.016.1046835
CAAAGTG352350.015.7738833
CGAGTAG54850.015.7566853
AGTAGCT185450.015.0795065
CCCAAAG461050.014.2984451
GTCGAGA46850.014.1010751
GCTGGGA693350.013.9741049
CCGAGTA70100.013.5320682
GAGTAGC139700.013.227884
TAGCTGG240300.013.0697127
GACTTGT144300.012.7089763
GCTAATT135700.012.4816261
AGTGCTG462900.012.2798586
TGATCAA152100.012.2413389
CCTATAT60350.011.7619161
ACTTGTG167100.011.5634424
GTAGCTG251150.011.1627636
GCTATAT59400.011.003491
Produced by FastQC (version 0.11.5)