FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19960893
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC286350.017.6685984
CGAGTAG57250.016.6930893
CCAAAGT331000.016.4531122
AAGTGCT336150.015.46911055
CAAAGTG357900.014.9219363
AGTAGCT184850.014.6355145
CCGAGTA69200.014.1150722
TAGCTGG231000.014.1148417
CCCAAAG454950.014.0472451
GCTGGGA699550.013.46034059
ACGTCGA27900.012.8515911
CGTCGAG41750.012.6297781
GAGTAGC139350.012.5562264
GATATAT80200.012.5358151
GACTTGT150900.012.4335553
GCTAATT132550.012.3054971
CCTATAT62800.012.2027521
CTACTCG53250.011.876742
AGTGCTG468100.011.7091676
TGATCAA158450.011.486239
Produced by FastQC (version 0.11.5)