FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21037711
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA27900.056.103451
CGTCGAG42000.037.602062
CGAGTAG71300.028.5070693
CCGAGTA81050.025.8639452
CTACTCG72250.024.8972822
GTCGAGA62350.024.8752733
GACTTGT230050.024.2585143
AGTAGCT308000.023.752475
TACTCGG80200.023.4891593
GAGTAGC198750.022.413144
TGATCAA249400.022.404739
GTAGCTG328100.022.016856
ACTTGTG264250.022.0032754
GCTGGGA789850.021.8061059
TAGCTGG358200.021.7682237
GTGATCA261400.021.4846278
AAAGTGC434300.020.9542684
CCCGAGT118600.020.304661
GCTACTC204700.019.9645461
CCAAAGT498000.019.9519042
Produced by FastQC (version 0.11.5)