FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21355743
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG28900.035.7668951
CGAGTAG68900.030.2094043
CTACTCG72250.027.9269892
GACTTGT220800.027.0618463
CCGAGTA79300.026.604812
TACTCGG81050.024.1958453
AGTAGCT308400.024.1271175
ACGTCGA18850.024.037811
ACTTGTG256650.024.0265224
GTGATCA256750.023.548358
TGATCAA257000.023.2500239
GAGTAGC200100.022.5375354
GTAGCTG329500.022.3672926
TAGCTGG360450.022.1356837
GCTGGGA792400.022.0503129
GAGACTT285000.021.7861751
GTCGAGA49250.021.5626492
AAAGTGC436250.021.551464
GCTACTC208750.021.4007571
AGACTTG281600.021.3950862
Produced by FastQC (version 0.11.5)