FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21253722
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA23200.048.8664441
CGTCGAG36100.038.4704781
CGAGTAG71650.029.6572273
CTACTCG72850.027.8075642
CCGAGTA80150.025.9818632
GACTTGT229050.025.2339553
TACTCGG81400.025.1477383
GTCGAGA56850.024.7941422
ACTTGTG261450.022.485774
AGTAGCT308450.022.4122475
TGATCAA260100.022.3299489
GTGATCA264900.021.9787548
GCTACTC210200.021.6411881
GAGTAGC197250.021.5453014
AAAGTGC436300.021.3480824
GTAGCTG328100.021.199356
GCTGGGA804350.021.0899099
TAGCTGG362850.020.6916947
GAGACTT297600.020.1902481
CCAAAGT510650.020.1127342
Produced by FastQC (version 0.11.5)