FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18463706
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC271150.018.8837664
CCAAAGT323450.017.561722
AAGTGCT317100.016.1475075
CAAAGTG339350.015.9538593
CGAGTAG45800.015.8627983
AGTAGCT178750.015.280185
CCCAAAG432550.015.2707911
GCTGGGA594700.014.8607189
TAGCTGG214450.014.8542897
CTACTCG50000.014.2532472
GCTACTC133150.013.3864381
GAGTAGC131200.013.0977144
CCGAGTA56150.013.0654142
AGTGCTG414700.013.02121456
GTAGCTG225600.012.1381636
TACTCGG59400.012.1133063
CCTATAT66650.011.8507091
GCTAATT133950.010.5408271
TTGGGAG335750.010.5070656
GTGCTGG570950.010.4978967
Produced by FastQC (version 0.11.5)