FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18215625
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC276750.019.6322634
CCAAAGT324100.018.4253042
CGTCGAG34150.017.614241
AAGTGCT316600.017.4263115
ACGTCGA23650.016.8577611
CAAAGTG342100.016.637913
CCCAAAG427300.015.910711
GCTGGGA602850.015.56659
CGAGTAG47300.014.3396323
CTACTCG49550.014.2532382
AGTAGCT177250.014.2416525
GTCGAGA43250.014.2276232
TAGCTGG217200.014.1333167
AGTGCTG419500.013.61850556
CGAGACT91250.012.9541841
GACTTGT154900.012.7299043
GCTATAG62150.012.7172581
GAGTAGC122900.012.6310094
TGATCAA158950.012.3621359
TACTCGG60400.012.2714643
Produced by FastQC (version 0.11.5)