FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18297633
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC272250.018.375934
CCAAAGT323850.017.1535322
CGTCGAG31150.017.0485361
AAGTGCT314050.016.0636355
CGAGTAG47100.015.7249463
CAAAGTG343750.015.7123443
CCCAAAG425150.015.2359341
AGTAGCT184950.014.9982845
CTACTCG47950.014.8642122
GCTGGGA601100.014.6066419
TAGCTGG224200.014.0195467
GCTAATT143800.013.4602291
CCGAGTA58800.012.9532732
GAGTAGC128200.012.8625834
AGTGCTG408200.012.8378916
GACTTGT150750.012.5144193
GCTATAT61650.012.4678381
GTCGAGA42050.012.4630862
CGAGACT90950.012.2159141
GTAGCTG230050.012.1187016
Produced by FastQC (version 0.11.5)