FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18063878
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC272700.018.4656374
CCAAAGT319900.016.8840482
AAGTGCT316900.015.8256675
CAAAGTG342800.015.4646173
CCCAAAG430750.015.0656911
CGAGTAG48250.014.9100163
GCTGGGA579350.014.3995689
AGTAGCT172800.014.18806655
TAGCTGG209750.014.0548327
GCTAATT132950.013.7809541
CTACTCG48250.013.6126262
CCGAGTA56050.013.4635482
AGTGCTG406150.013.0026326
GCTACTC126600.012.9807711
GCTATAT62850.012.3504871
CCTATAT67950.011.938091
GAGTAGC124800.011.8641184
GTAGCTG221950.011.6765536
TACTCGG60250.011.5926083
GTGCTGG543350.010.37557
Produced by FastQC (version 0.11.5)