FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18063878
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA25950.023.9696621
AAAGTGC270900.018.9081974
CGTCGAG37700.018.5382161
CCAAAGT322950.017.5267452
CGAGTAG47700.017.433373
AGTAGCT174350.016.914215
AAGTGCT312150.016.454525
CAAAGTG335900.016.247773
TAGCTGG214700.016.1114397
CCCAAAG425400.015.2789931
GCTGGGA595050.015.2669929
CCGAGTA59850.014.7115352
GAGTAGC125600.014.5769554
CTACTCG47200.014.5089392
GCTACTC127850.014.2128741
GTCGAGA49050.014.10417652
GTAGCTG224100.013.5022846
TACTCGG56650.012.8288043
AGTGCTG411800.012.6424466
GACTTGT149650.011.9540773
Produced by FastQC (version 0.11.5)