FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17552320
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC274600.019.203564
CCAAAGT322100.017.5289442
AAGTGCT324600.016.4457555
CAAAGTG340050.016.0664163
CTACTCG51150.015.7295292
AGTAGCT178300.015.207595
CCCAAAG425550.015.0941671
GCTGGGA624000.014.9750139
CGAGTAG50900.014.8383923
TAGCTGG214100.014.7439377
TACTCGG58500.014.2379353
AGTGCTG424800.013.2151416
GCTACTC127750.012.9845021
GAGTAGC131550.012.3423734
CCGAGTA62400.012.3281632
GCTAATT132400.012.3152251
GTAGCTG230950.012.1995576
GATATAT75750.011.0887531
CCTATAT63150.010.6186321
GTGCTGG591450.010.4952667
Produced by FastQC (version 0.11.5)