FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19773218
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC316450.018.8664364
CCAAAGT371200.017.1059132
AAGTGCT362650.016.9816445
AGTAGCT193750.016.6288955
CAAAGTG385350.016.325023
CGAGTAG56450.016.2723623
CTGTCTA135450.015.957854145
CCCAAAG491000.015.4246561
GCTGGGA706450.014.7752219
CTACTCG57400.014.6768512
TAGCTGG247550.014.5040747
GCTACTC148400.013.85018351
GAGTAGC140900.013.630314
CCGAGTA68650.013.4887122
AGTGCTG478350.013.3685746
GTAGCTG252150.012.8324436
GCTAATT155950.012.8222421
TACTCGG72050.011.9764573
GCTACTT131300.010.93125251
CCTATAG66650.010.6627051
Produced by FastQC (version 0.11.5)